Recombinant Human/Mouse/Rat Irisin

Category:
Alternative Names：Fibronectin type III domain containing 5 Protein, Fibronectin type III domain-containing protein 5 Protein, Fibronectin type III repeat containing protein 2 Protein, Fibronectin type III repeat-containing protein 2 Protein, FNDC 5 Protein, Fndc5 Protein, FNDC5_HUMAN Protein, FRCP2 Protein, irisin Protein
Catalogue No.：P8693Uniprot ID：Q8NAU1
Host：HEK293 cellsExpression Region：32-143
Mol. Weight：17-25kDaTags：C-terminal His Tag
Manual MSDS
  • SPECIFICATIONS
Product Name
Recombinant Human/Mouse/Rat Irisin
Catalogue No.
P8693
Species
Human/Mouse/Rat
Host
HEK293 cells
Expression Region
32-143
Mol. Weight
17-25kDa
Tags
C-terminal His Tag
Purity
Greater than 90% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured by its ability to induce p38 MAPK activation in 3T3?L1 mouse embryonic fibroblast adipose-like cells. 1 μg/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
Fibronectin type III domain containing 5 Protein, Fibronectin type III domain-containing protein 5 Protein, Fibronectin type III repeat containing protein 2 Protein, Fibronectin type III repeat-containing protein 2 Protein, FNDC 5 Protein, Fndc5 Protein, FNDC5_HUMAN Protein, FRCP2 Protein, irisin Protein
SDS-PAGE
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